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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Diet-induced obesity and low testosterone increase neuroinflammation and impair neural function

Figure 5

High-fat diet-derived glia cultures yield reduced neurite outgrowths. Primary cortical neurons were plated either by themselves or on confluent primary glial cultures derived from the cortices of sham GDX (Sham), GDX, and GDX mice with testosterone treatment (GDX + T) in both control-diet and high-fat diet. Top panels show representative pictures of neurite outgrowths on neurons co-cultured with (A) control diet- and (B) high-fat diet-derived glia. Quantitative graphs show mean (C) number of neurites and (E) length of neurites per neuron across treatment groups in the neuron-glia co-cultures. Quantitative graphs show mean (D) number of neurites and (F) length of neurites per neuron in conditioned glial media from each group. Data show mean values (±SEM) of a representative experiment. * P ≤0.05 between diet groups; ** P ≤0.05 between hormone groups; N = 3.

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