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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Neisseria meningitidiselicits a pro-inflammatory response involving IκBζ in a human blood-cerebrospinal fluid barrier model

Figure 3

qPCR analysis of selected genes and Nm -induced cytokine production in infected HIBCPP cells. (A) Expression of genes coding for IκBζ, IL6, CXCL1-3, IL8, TNFα, and ADM was quantified by qPCR and a genorm-based approach with simultaneous normalization to β-actin and GAPDH (n = 6). * (significant; P <0.05), ** (highly significant; P <0.01), *** (extremely significant; P <0.001); when cells treated with α14, MC58, or MC58siaD-, respectively, were compared to the uninfected control. (B) Concentrations of IL6, G-CSF, GM-CSF, CXCL1-3, IL8, and TNFα were measured in HIBCPP cell culture supernatants after infection. In all experiments, cells were treated for 4 h with bacteria followed by antibiotic killing of the bacteria and further incubation up to 24 h. Bacteria were applied at a multiplicity of infection of 10, untreated cells served as a control.

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