Figure 4
α 1 -antitrypsin (A1AT) reduces Aβ-induced release of TNF-α. ELISA measurements were performed to detect the amount of the pro-inflammatory cytokines TNF-α (A) and IL-6 (B) in supernatants of Aβ1-42 oligomer and/or A1AT co-treated primary microglial cells 24 hours after treatment. Results were normalized to basal cytokine levels of untreated cells. Experiments were performed at least three times independently. Means and standard deviations of the mean of three independent experiments are shown (*P value ≤0.05, ***P value ≤0.001, n.s., not significant).