Figure 4

Polyinosinic:polycytidylic acid (poly(I:C)) and isoflavone effect on skin gene expression of inflammatory mediators. Mice were provided with either low or high isoflavone diet ad libitum. Mice were injected intraperitoneally (ip) with 20 mg/kg poly(I:C) and were forced to swim for 15 minutes. Following this, they were individually placed into specific cages and locomotor activity was monitored overnight. The next day, skin samples were collected and analyzed for TNF-α (Figure 4 A), IL-6 (Figure 4 B), KC (Figure 4 C) and CCL4 (Figure 4 D) skin gene expression using qPCR.