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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Infliximab reduces Zaprinast-induced retinal degeneration in cultures of porcine retina

Figure 2

Infliximab prevents Zaprinast-induced cell death in cultured porcine retina. Retinal explants were incubated with dimethyl sulfoxide (DMSO), Zaprinast and Infliximab alone or combined with Zaprinast as described in Methods. Confocal laser scanning micrographs of retinal sections showing TUNEL-stained sections visualizing apoptotic photoreceptors (pink) (A), cleaved caspase-3 positive cells (red) (B) and PAR accumulation (pink) (C) in SYTOX Green-counterstained retinal sections. Scale bar: 50 μM. (D) Bar graphs showing the quantification of TUNEL, cleaved caspase-3 and PAR accumulation. Values are the mean ± SEM of seven different cultures. Values that are significantly different are indicated by asterisks *P <0.05; **P <0.01; ***P <0.001 (Kruskal-Wallis, Dunn’s post-test). C: control; Z100: 100 μM Zaprinast; INF: 2’μg/mL Infliximab; Z100 + INF: 100’μM Zaprinast with 2’μg/mL Infliximab. TUNEL, terminal deoxynucleotidil transferase dUTP nick and labeling.

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