Figure 9
From: Molecular profile of cochlear immunity in the resident cells of the organ of Corti
Typical images of Tlr3 immunoreactivity in the sensory epithelium representing the cochlear section at the distance approximately 70% from the apex. The tissues were examined 1 day after noise exposure. (A) Tlr3 immunoreactivity. (B) Propidium iodide staining of the same tissue. (C) Superimposed (A) and (B). Arrows show the sensory cells with the increased Tlr3 immunoreactivity. (D) High magnification view of sensory cells in a section of the basal turn of the cochlea. Arrows indicate an increase in Tlr3 immunoreactivity in outer hair cells with nuclear fragments. The double-arrow indicates an outer hair cell area that has no nuclear staining, suggest degradation of the nucleus. These images show that damaged outer hair cells display an increase in Tlr3 protein immunoreactivity. Bar in (B) =25 μm and Bar in (D) =10 μm. IHC: inner hair cells. OHC: outer hair cells.