Isolation of peritoneal macrophages (φ) and microglia (μglia) from adult mice shows enrichment of CCR2 and CX3CR1 cell populations, and loss of p38α in the isolated macrophages but not in the microglia fraction. RNA was extracted from peritoneal macrophages or microglia isolated from cortical tissue using a discontinuous Percoll gradient. qRT-PCR using TaqMAN Gene Expression Assays was used to determine CCR2, CX3CR1, and p38α (MAPK14) expression in the isolated cell populations. Data are presented as fold change of wild-type (WT) macrophages. The data are representative of two independent experiments.