Figure 3

Western blotting analysis of VEGF, AQP4, GFAP, and NKCC1 protein expression levels in the olfactory bulb of the C group (lane 1); SI + S group, at 0.5 h (lane 2), 3 h (lane 3), 24 h (lane 4), and 72 h (lane 5); and SI + AG group, at 24 h (lane 6) and 72 h (lane 7). (A) VEGF (21 kDa), AQP4 (34 kDa), GFAP (45 kDa), NKCC1 (170 kDa), and β-actin (42 kDa) immunoreactive bands. Bar graphs represent optical density (mean ± standard deviation) of (B) VEGF, (C) AQP4, (D) GFAP, and (E) NKCC1 normalized to β-actin for C, SI + S at 0.5, 3, 24, and 72 h and SI + AG at 24 h and 72 h (* P <0.05; **P <0.01). (B) VEGF shows a steady increase in the SI + S group at 0.5, 3, 24, and 72 h, as compared with that of the control with recovery at 72 h in the SI + AG group, as compared with 72 h. (C) AQP4 shows an acute increase at 0.5, 3, 24, and 72 h in the SI + S group, as compared with the control. (D) GFAP shows a significant increase at 24 and 72 h in the SI + S group, as compared with the control, with recovery at 72 h in the SI + AG group, as compared with 72 h. (E) NKCC1 shows a decrease at 0.5 h but rises after this peaking at 24 h in the SI + S group, as compared with the control. (* P <0.05, ** P <0.01).