Figure 4

Western blotting analysis of iNOS, eNOS, and nNOS protein expression levels in the olfactory bulb of the C group (lane 1); the SI + S group at 0.5 h (lane 2), 3 h (lane 3), 24 h (lane 4) and 72 h (lane 5); and the SI + AG group at 24 h (lane 6) and 72 h (lane 7). (A) iNOS (130 kDa), eNOS (140 kDa), nNOS (155 kDa), and β-actin (42 kDa) immunoreactive bands. Bar graphs represent optical density (mean ± standard deviation) of (B) iNOS, (C) eNOS, and (D) nNOS normalized to β-actin for C, SI + S at 0.5, 3, 24, and 72 h and SI + AG at 24 and 72 h (* P <0.05; ** P <0.01). (B) iNOS shows a significant increase in the SI + S group at 3, 24, and 72 h, as compared with the C group, with recovery at 72 h in the SI + AG group, as compared with the SI + S group at 72 h. (C) eNOS shows an increase in the SI + S group at 24 and 72 h, as compared with the C group. Administration of aminoguanidine did not affect its expression level significantly. (D) nNOS shows a significant increase in the SI + S group at 3 and 24 h, as compared with the control. Aminoguanidine administration did not affect its expression level significantly.