Figure 5

VEGF (red) and GFAP (green) immunopositive astrocytes in the external plexiform layer, mitral cell layer, and internal plexiform layer in the olfactory bulb of the C group (A-C), SI + S group at 24 and 72 h (D-I), and SI + AG group at 72 h (J-L). In the control, moderate VEGF immunofluorescence is detected in GFAP-labeled astrocytes. In the SI + S group, VEGF immunofluorescence is evidently enhanced in hypertrophic astrocytes at 24 and 72 h (D-I), notably at 24 h (E). Colocalized expression of VEGF with GFAP immunoreactive astrocytes (arrows) and the lumen of blood vessels (asterisks) can be seen in C, F I, and L. Immunoreactivity for both VEGF and GFAP is attenuated in the SI + AG group at 72 h, as compared with the SI + S group at corresponding time point (J-L). Scale bar =20.0 μm.