Figure 8

NKCC1 (red) and GFAP (green) immunopositive astrocytes in the bulb tissue of the control group (A-C), the SI + S group at 24 and 72 h (D-I), and the SI + AG group at 72 h (J-L). In the C group, only sporadic astrocytes exhibit moderate NKCC1 immunofluorescence. In the SI + S group, it is evidently enhanced in hypertrophic astrocytes at 24 and 72 h (D-I), being more pronounced at 24 h (E). Colocalized expression of NKCC1 with GFAP immunoreactive astrocytes (arrows) can be seen in C, F, I and L. Immunoreactivity for both NKCC1 and GFAP is attenuated at 72 h in the SI + AG group, as compared with the SI + S group at 72 h (J-L). Scale bar =20.0 μm.