Combustion smoke-induced inflammation in the olfactory bulb of adult rats

Table 1 Number of rats used in different experiments

	Control	0.5 h	3 h	24 h	72 h	Day 7	Day 14	Total
Inflammatory cytokines assay	3			SI + S 3				9
Inflammatory cytokines assay	3			SI + AG 3				9
Western blotting	4	SI + S 4	+ S 4	SI + S 4	SI + S 4			28
Western blotting	4	SI + S 4	+ S 4	SI + AG 4	SI + AG 4			28
Double immunofluorescence	4			SI + S 4	SI + S 4	+ S 4	+ S 4	28
Double immunofluorescence	4			SI + AG 4	SI + AG 4	+ S 4	+ S 4	28
Nitrite assay	4	SI + S 4	SI + S 4	SI + S 4	SI + S 4			44
			SI + S 4	SI + AG 4	SI + AG 4
			SI + AG 4	SI + N 4	SI + N 4
			SI + N 4	SI + N 4	SI + N 4
Glutamate assay	4	SI + S 4	SI + S 4	SI + S 4	SI + S 4			28
Glutamate assay	4	SI + S 4	SI + S 4	SI + AG 4	SI + AG 4			28
Assessment of vascular permeability	3			SI + S 3				9
Assessment of vascular permeability	3			SI + AG 3				9
Detection of apoptosis	3				SI + S 3			9
Detection of apoptosis	3				SI + AG 3			9
Total								155

SI + AG, smoke inhalation plus aminoguanidine; SI + N, smoke inhalation plus L-NAME; SI + S, smoke inhalation plus saline.

ISSN: 1742-2094