Figure 6

Metformin promoted phosphorylation of AMPK and reduced ICAM-1 expression in vitro in an AMPK-dependent manner. (A) p-AMPK and AMPK expression and quantification at 30, 60 and 120 minutes after metformin treatment in vitro in normal conditions. (B) p-AMPK and AMPK expression and quantification in control, 1, 4 and 24 hours in reoxygenation group in the presence of metformin in vitro. (C) Fold change of ICAM-1 expression in mRNA level 1, 4 and 24 hours after reoxygenation in saline- and metformin-treated group. (D) ICAM-1 expression in mRNA after metformin and AMPK inhibitor and compound C treatment in the oxygen glucose deprivation (OGD) model. (E) ICAM-1 expression and quantification after metformin, AMPK inhibitor and compound C treatment in OGD model. (F) p-AMPK and AMPK expression and quantification after metformin and compound C treatment in OGD model. (G) p-NF-κB and NF-κB expression and quantification after metformin and compound C treatment in OGD model. Data were mean ± SD, *P < 0.05, **P < 0.01. Representative results from three independent experiments are shown. Com. C = compound C.