Enhanced phosphorylation of Janus kinase 2 (JAK2) and up-regulation of JAK2-related genes in the spinal cord of mSOD1
mice in the late stage of disease. (A) Quantitative RT-PCR analyses of spinal cords of mSOD1G93A mice (70 days and 130 days old) were performed (n = 3 to 4 for each group). Expression levels of INF-γ, Il-6, Il-12a, and GM-CSF were significantly elevated in 130-day-old mSOD1G93A mice relative to those in 70-day-old mice. Data are expressed as means ± SEM. *P < 0.05, Mann-Whitney U-test. (B) Immunohistochemical analysis showed enhanced phosphorylation of JAK2 in the spinal cord of late-stage mSOD1G93A mouse compared with the spinal cord of pre-onset-stage mSDO1 mouse. Scale bar = 100 μm. Data are representative of three animals. (C) Sections of 130-day-old mSOD1G93A mouse spinal cord were co-stained with Cy5-conjugated anti-phosphorylated JAK2 antibodies and FITC-conjugated tomato lectin. Scale bar = 100 μm. Data are representative of three animals.