Figure 3
From: The role of aquaporin 4 in apoptosis after intracerebral hemorrhage
Representative TUNEL-positive cells with morphologies suggestive of apoptosis. (A) Left, AQP4-/- mice; right, AQP4+/+ mice. Colocalized cells (yellow, arrows) by TUNEL (green) and NeuN (red) fluorescence represent the cell type as neuron, while those double labeled by TUNEL (green) and GFAP (red) fluorescence are astrocytes. Magnified images of the regions of interest are indicated by a white rectangle in the frame at the lower right corner. Scale bar: 500 μm (left graph); 200 μm (right graph). (B) Quantitative analysis shows more apoptosis-positive cells (both neurons and astrocytes) 3 days after ICH in AQP4+/+ mice than in AQP4-/- mice (n =6, * P <0.05). GFAP, glial fibrillary acidic protein; ICH, intracerebral hemorrhage; NeuN, neuron-specific nuclear protein; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.