Table 1 Influence of different protein kinase C activators and inhibitors on neocortical layer 5 neurons
Control versus 4β-PMA | Control versus Bryostatin1 | Control versus IFN-β | Control versus GF109203X + IFN-β | calphostin C versus calphostin C + IFN-β | |
|---|---|---|---|---|---|
Experiments (n) | 17 | 7 | 8 | 9 | 12 |
Input resistance (MΩ) | 74.8 ± 9.8 vs 105.1 ± 13.6 | 69.13 ± 15.3 vs 87.3 ± 25.5 | 76.5 ± 9.7 vs 89.1 ± 11.1 | 68.1 ± 11.2 vs 73.3 ± 10 | 67.7 ± 10.3 vs 74.2 ± 11.9 |
(P < 0.01†) | (P < 0.05†) | (P < 0.05#) | (P = 0.4#) | (P = 0.9†) | |
Rheobase (pA) | 124.1 ± 16 vs 103.5 ± 14.1 | 120.8 ± 20 vs 105.6 ± 17 | 185.2 ± 28 vs 146.9 ± 20 | 148.3 ± 29 vs 140.6 ± 24 | 170 ± 19 vs 200 ± 17 |
(P < 0.05#) | (P = 0.2†) | (P < 0.05#) | (P = 0.7#) | (P = 0.1#) | |
F-I slope (Hz/nA) | 76 ± 4.9 vs 96 ± 7.5 | 81 ± 10 vs 103 ± 11 | 47.7 ± 3.9 vs 57.9 ± 5.3 | 91.1 ± 9.9 vs 87.8 ± 6.8 | 100 ± 11.8 vs 104 ± 9.6 |
(P < 0.01†) | (P <0.05†) | (P < 0.05#) | (P = 0.7#) | (P = 0.5#) | |
After-hyperpolarization (mV) | 5.8 ± 0.3 vs 2.7 ± 0.3 | 6.4 ± 0.64 vs 3.6 ± 0.42 | 5 ± 0.25 vs 3.4 ± 0.5 | 3.7 ± 0.44 vs 3.1 ± 0.4 | 3.9 ± 0.5 vs 2.6 ± 0.4 |
(P < 0.005#) | (P <0.05†) | (P < 0.01#) | (P = 0.1#) | (P < 0.005#) | |
Series resistance (MΩ) | 7.99 ± 0.27 vs 8.5 ± 0.47 | 8.89 ± 0.56 vs 8.94 ± 0.67 | 7.8 ± 0.3 vs 8.1 ± 0.4 | 8.5 ± 0.6 vs 8.2 ± 0.6 | 8.9 ± 0.5 vs 12.8 ± 2.9 |
(P = 0.3#) | (P = 1†) | (P = 0.2†) | (P = 0.7#) | (P = 0.8†) |