Cytokine profiles by peripheral blood monocytes are associated with changes in behavioral symptoms following immune insults in a subset of ASD subjects: an inflammatory subtype?

Table 5 IL-6 production by purified peripheral blood monocytes in the study groups

Study group	Without stimuli [pg/ml]	LPS	Zymosan
ASD-IS
- Flare	4541.7 ± 1332.8^a	20662 ± 6181	936.6 ± 575.1^b
- Non-Flare	3136.6 ± 1276.7	19420 ± 7051	1235.4 ± 1217
ASD/NFA	3682.7 ± 1613.3	17708 ± 10952	1352.4 ± 831.6
ASD/non-NFA	3827.3 ± 1467.4	18632 ± 10615	1281.7 ± 1068.5
Non-ASD/NFA	4029.8 ± 1246.1	20040 ± 6837	1334.0 ± 1031.4
Normal control	3102.8 ± 1225.4	17919 ± 10845	1716.1 ± 942.7
PANS
- Flare	4005.0 ± 1512.2	22455 ± 9097	1123.9 ± 1487.5
- Non-Flare	3726.9 ± 1143.0	19663 ± 9718	871.6 ± 1013.0

^aSpontaneous IL-6 production by `flare’ ASD-IS cells was higher than `non-flare’ cells in matched pair analysis, as shown in Figure 4 and also higher than those produced by ASD/NFA and normal control cells.
^bIL-6 production by `flare’ ASD-IS cells in response to zymosan was lower than those produced by normal control cells (P <0.02) and also lower than the `non-flare’ ASD-IS cells in matched pair analysis (P <0.01).
^c Abbreviations used; ASD, autism spectrum disorder; ASD-IS, ASD-inflammatory subtype; IL, interleukin; LPS, lipopolysaccharide; NFA, non-IgE mediated food allergy; PANS, pediatric acute-onset neuropsychiatric syndrome.

ISSN: 1742-2094