Figure 2

Relative affinity distributions. Relative affinity (RA) of anti-Epstein Barr nuclear antigen 1 (EBNA-1) IgG (white boxes) and of anti-viral capsid antigen (VCA) IgG (gray boxes) values obtained with increasing concentrations of sodium thiocyanate (NaSCN) in cerebrospinal fluid (CSF) and serum from 50 relapsing-remitting multiple sclerosis (RRMS), 50 other inflammatory neurological disease (OIND) and 50 noninflammatory neurological disease (NIND) patients. CSF and serum RA1 values were higher for anti-EBNA-1 IgG than for anti-VCA IgG in RRMS (panels A and D; Mann- Whitney; P <0.0001), OIND (panels B and E; Mann- Whitney; P <0.0001) and NIND (panels C and F; Mann- Whitney; P <0.0001). CSF RA2 values were more elevated for anti-VCA IgG than for anti-EBNA-1 IgG in OIND (panel B; Mann- Whitney; P <0.01) and NIND (panel C; Mann- Whitney; P <0.05). Serum RA2 values were greater for anti-VCA IgG than for anti-EBNA-1 IgG in RRMS (panel D; Mann- Whitney; P <0.01), OIND (panel E; Mann- Whitney; P <0.05) and NIND (panel F; Mann- Whitney; P <0.01). CSF RA3 values were higher for anti-VCA IgG than for anti-EBNA-1 IgG in RRMS (panel A; Mann- Whitney; P <0.001) and OIND (panel B; Mann- Whitney; P <0.001). Serum RA3 values were more increased for anti-VCA IgG than for anti-EBNA-1 IgG in RRMS (panel D; Mann- Whitney; P <0.05), OIND (panel E; Mann- Whitney; P <0.0001) and NIND (panel F; Mann- Whitney; P <0.001). The boundaries of the boxes represent the 25th to 75th quartile. The line within the box indicates the median. The vertical lines above and below the box correspond to the highest and lowest values, excluding outliers. RA, percentage of IgG removed by: 1.25 M NaSCN (RA1), 2.5 M NaSCN (RA2), 5 M NaSCN (RA3), and percentage of IgG remaining after treatment with 5 M NaSCN (RA4).