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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Inhibition of metabotropic glutamate receptor 5 induces cellular stress through pertussis toxin-sensitive Gi-proteins in murine BV-2 microglia cells

Figure 1

Increased cellular stress and inflammatory mediators upon inhibition of mGluR5 in BV-2 cells. BV-2 cells were treated for 24 hours with 100 μM of either CHPG or MPEP. (A) Intracellular reactive oxygen species (ROS) levels were determined using dihydroethidium (DHE). (B) Mitochondrial mass was measured using MitoTracker®Red CMXRos. (C) Mitochondrial superoxide levels were determined using MitoSOX Red. The expression of inducible nitric oxide synthase (iNOS) mRNA (D) and IL-6 mRNA (E) were measured by quantitative PCR. Results are expressed relative to the values of the house-keeping control GAPDH. (F) IL-6 protein levels were determined by ELISA. Results represent mean ± SD of three independent experiments. Significance was analyzed by one-way ANOVA followed by Tukey’s test. ***P <0.005. CHPG, (RS)-2-chloro-5-hydroxyphenylglycine; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MPEP, 2-methyl-6-(phenylethynyl)-pyridine.

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Journal of Neuroinflammation

ISSN: 1742-2094