Figure 2
TNF-α mRNA in the injured cortex measured using quantitative real-time PCR (A-C) and TNF-α protein concentration using an enzyme-linked immunosorbent assay (D). (A) TNF-α mRNA at 6 hours, 2 and 3 days after CCI injury compared to craniotomy (control), *P < 0.05 and ***P < 0.001. (B) TNF-α mRNA at 1 day after CCI injury in wild-type mice treated with a vehicle solution (vehicle), cannabinoid receptor type-2 (CB2) agonist (0-1966) or CB2 receptor antagonist (SR144528) compared to vehicle control, ***P < 0.001. (C) TNF-α mRNA at 1 day after CCI injury in CB2 knockout (CB2 KO) and wild-type mice compared to control, ***P < 0.001, and ##P < 0.01 compared to wild-type mice. (D) TNF-α protein concentration at 1 day post-CCI in vehicle-treated and CB2 agonist-treated (JWH-133) mice compared to control and vehicle, respectively, **P < 0.01.