Effects of ATB-346 on interleukin (IL)-1β expression in ionized calcium binding adaptor molecule (Iba)1 positive cells. Brain tissue was double-stained with antibodies against Iba1 (green) and IL-1β (red). Microglial cells (Iba1+ cells) expressed IL-1β in TBI and TBZ panels. A considerable reduction of IL-1β expression was present in naproxen panels; at least, ATB-346 markedly reduced TNFα and IL-1β expressions (ATB-346 panels). The red spots indicate the co-localizations (merged). All images were digitalized at 600 dpi.