Figure 1

Analysis of structural damage and cell death in the choroid plexus. Rabbit pups with IVH + PHVD (B, D, F) or sham controls (A, C, E) were euthanized at 72 hours of age and the brains were removed from the skulls, sectioned at the level of the midseptal nucleus and placed in formaldehyde for subsequent histochemical analysis with H&E (A and B) and IHC analysis against cleaved caspase-3 (E and F) as described in Materials and Methods. For EM-IHC (C and D), the choroid plexus from pups with IVH + PHVD and sham controls at 72 hours was fixed and prepared as described in the Materials and Methods and observed in a Jeol JEM 1230 electron microscope. Recessed picture display mitochondrial structure. P = periventricular tissue, CP = choroid plexus, V = ventricle, Vi = villi, M = mitochondria. Arrow illustrates cleaved caspase-3 positive staining. Scale bar in (A) indicate 50 μm (is applicable for panels (A) and (B)), in (C) indicate 500 nm (is applicable for (C) and (D)) and (E) indicate 20 μm (is applicable for (E) and (F)). IHC, immunohistochemistry; IVH, intraventricular hemorrhage; PHVD, post-hemorrhagic ventricular dilatation.