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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Extracellular hemoglobin - mediator of inflammation and cell death in the choroid plexus following preterm intraventricular hemorrhage

Figure 7

Hp reduces Hb-induced cellular activation, inflammatory response and oxidative stress in choroid plexus epithelial cell cultures. mRNA expression of NF-Κβ (A), MCP-1 (B), IL-8 (C), IL-1β (D), IL-6 (E) and HO-1 (F) in HCPEpiC cells, exposed to 10% (of cell culture medium volume) cerebrospinal fluid (CSF) from preterm infants with IVH or 10 μM metHb with or without the addition of 1.0 mg/ml Hp, for 4 and 24 hours was determined using real-time PCR as described in Materials and Methods. The mRNA expression of all respective genes was normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is given as fold change. The fold-change values were calculated by normalizing against control samples from untreated cells. Results are from triplicate experiments and presented as mean ± SEM. Differences between the respective exposures versus control for respective time point were analyzed using ANOVA post hoc Bonferroni. *P < 0.05, **P < 0.01.

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