Skip to main content
Figure 3 | Journal of Neuroinflammation

Figure 3

From: Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies

Figure 3

Neutralization of exogenous quinolinic acid (QUIN) with an anti-QUIN monoclonal antibody (mAb). The QUIN toxicity assay was investigated in (a) N19 and (b) N20.1 cells treated with various concentration of QUIN (0, 0.05, 0.1, 0.2, 0.5, 1, 2 and 4 μM) for 24 hours, 48 hours and 72 hours. Lactate dehydrogenase (LDH) activity in culture supernatant was determined. The results were analyzed using linear regression analysis. (c) The schematic diagram showed the treatment conditions of QUIN and QUIN mAb on oligodendroglial cells. The effect of QUIN mAb on QUIN toxicity was examined in (d) N19 and (e) N20.1 cells. The levels of LDH released by oligodendroglial cell lines after 72 hours of incubation with 0.5 μM (N19) and 1 μM (N20.1) of QUIN and varying concentrations of QUIN mAb (30 minutes). The study was performed in triplicate and the error bars indicate SE. ***P < 0.001.

Back to article page