Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies

Table 1 Primer sequences for qRT-PCR

Gene	ID	Accession number	Primer sequence
Indoleamine 2,3-dioxygenase	IDO1	NM_008324	FWD	TGT GAA TGG TCT GGT CTC
Indoleamine 2,3-dioxygenase	IDO1	NM_008324	REV	CTG TGC CCT GAT AGA AGT
Tryptophan 2,3-dioxygenase	TDO2	BC018390.1	FWD	TGC TCA AGG TGA TAG CTC GGA
Tryptophan 2,3-dioxygenase	TDO2	BC018390.1	REV	AGG AGC TTG AAG ATG ACC ACC A
Kynurenine aminotransferase 1	KAT1	NM_172404	FWD	GCT TTT CAG CAG GCT ACC AC
Kynurenine aminotransferase 1	KAT1	NM_172404	REV	CCA CTG TCA CCA GCA CAT TC
Kynurenine aminotransferase 2	KAT2	AF072376	FWD	GAA CTT CTG TCC TGG CTA A
Kynurenine aminotransferase 2	KAT2	AF072376	REV	CTT GAT TGG GTG GGT AGT
Kynureninase	KYNU	NM_027552	FWD	GAG CAG AGG AGC GTG GCT GC
Kynureninase	KYNU	NM_027552	REV	GAA CAG GGG CCA CGC GGA TG
3-hydroxyanthranilate 3,4-dioxygenase	3HAO	NM_025325	FWD	TTC AGC CTC ATT GCA TCT
3-hydroxyanthranilate 3,4-dioxygenase	3HAO	NM_025325	REV	GAC AGT GTA GGG CTA TGG
Kynurenine 3-monoxygenase	KMO	NM_133809.1	FWD	GGT CGC CTT CAC CAG AAT AA
Kynurenine 3-monoxygenase	KMO	NM_133809.1	REV	ATC CAG GCA GGT CTT CTC AA
Aminocarboxymuconate semialdehyde decarboxylase	ACMSD	NM_001033041	FWD	GAA TAA ATG CTG ACC CAA CA
Aminocarboxymuconate semialdehyde decarboxylase	ACMSD	NM_001033041	REV	TTC ATC CAT CCT TCC AGA C
Quinolinate phosphoribosyl transferase	QPRT	NM_133686.1	FWD	CTG CTC CAA GTC ACC ATG
Quinolinate phosphoribosyl transferase	QPRT	NM_133686.1	REV	CAG AAC CCC AGG AGA TTT
Ribosomal protein L13	RPL13	NM_016738.5	FWD	GAG GTC GGG TGG AAG TAC CA
Ribosomal protein L13	RPL13	NM_016738.5	REV	TGC ATC TTG GCC TTT TCC TT

ISSN: 1742-2094