Local depletion of regulatory T cells (Tregs) induces spontaneous autoimmune disease. (A) Incidence and severity of experimental autoimmune uveoretinitis (EAU) in the eyes of mice following anterior chamber of the eye (AC) injections of diphtheria toxin (DTx) or saline. Amount, timing, and duration of AC injection protocols are indicated. Statistical set A compares βgal-FDG-BG2 mice treated with saline or DTx, P values determined by Fisher’s exact test. Statistical set B compares mice with or without BG2 T cells following DTx treatment, P values determined by Fisher’s exact test. (B) Representative histology of eyes from βgal-FDG-BG2 mice that received DTx or saline into the right AC. (C) Fluorescence-activated cell sorting (FACS) analysis of peripheral blood mononuclear cells showing AC injection of DTx does not alter circulating Treg levels. Results are given as mean ± SD with P values determined by t test.