Systemic depletion of regulatory T cell (Tregs) induces spontaneous autoimmune disease in the retina. (A) Analysis of circulating Tregs levels following low and high dose systemic depletion protocols. Results are given as mean ± SD with P values determined by t test. (B) Incidence and severity of experimental autoimmune uveoretinitis (EAU) following systemic injections of diphtheria toxin (DTx). Amount, timing, and duration of systemic DTx injection protocols are indicated. P values determined by Fisher’s exact test. Analysis of EAU and Treg levels were done at 21 days post-initial DTx injection.