Retinal beta-galactosidase (βgal) expression induced immunological unresponsiveness. (A) Legend and time course of the experiments. Mice received 250 ng diphtheria toxin (DTx) and/or βgal (20 μg) or saline as indicated. Retinal T cell analysis was done by fluorescence-activated cell sorting (FACS) 3 days post-injection to the anterior chamber of the eye (AC). (B-E) Number of T cells per retina. T cells were analyzed as being effector (GFP−) or regulatory (GFP+) and βgal-specific (Vα11+) or nonspecific (Vα11−). Results are given as mean ± SD with P values determined by t test comparing FDG-BG2 to βgal-FDG-BG2 mice for each type of T cell with significant differences indicated by bracket.