Figure 7

Interphotoreceptor retinoid binding protein (IRBP)-induced autoimmune disease is enhanced in retinas depleted of regulatory T cells (Tregs). (A) Incidence and severity of experimental autoimmune uveoretinitis (EAU) in IRBP-immunized only FDG mice and in right and left eyes of FDG mice that were IRBP-immunized and given diphtheria toxin (DTx) into the right anterior chamber of the eye (AC). Mice were given 25 ng of DTx 3 times per week for 3 weeks starting the day of immunization. Retinas were analyzed for EAU at 21 days post-immunization. (B) Histology of right and left eyes from IRBP-immunized mice given DTx into the right AC. (C) Analysis of blood for Tregs in naïve (day 0) and DTx-treated FDG mice (day 21). For disease scores, mean and SD are given, P values were determined by Fisher’s exact test for incidence and Mann–Whitney test for severity. For blood analysis, results are given as mean ± SD with P value determined by t test.