Figure 7
From: Antibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia
Results from preadsorption experiments. Preadsorption of the patient serum with purified rat ITPR1 or an extract of HEK293 cells expressing murine ITPR1 resulted in complete loss of binding to cerebellar tissue sections in an indirect immunofluorescence assay (A, B, E). In contrast, binding of anti-Ca/ARHGAP26-positive sera was not affected by preadsorption with ITPR1 (C, D). Preadsorption of anti-ITPR1-positive patient serum with full-length human ARHGAP26 (not shown) or with an extract of mock-transfected HEK293 (F) did not affect binding to PCs.