Figure 4

Pre-treatment with vitamin D3 significantly decreased IL-6 and CXCL 1 in cerebellum homogenates at early infection. (A) IL-6, (B) IL-10, (C) CXCL 1 (KC), and (D) CXCL 2 (MIP-2) were measured 20 h after intracerebral E. coli K1 infection (9,000 CFU/mouse). Each symbol represents an individual mouse (n = 17 per group). Horizontal bars indicate median values. Statistical analysis was performed using the Kruskal-Wallis test and corrected for repeated testing with the Bonferroni method (*P < 0.05, ns: not significant). Filled circles, standard vitamin D (S-VitD) diet-fed mice (vitamin D3 concentration 1,500 IU/kg food); filled squares, low vitamin D (L-VitD) diet-fed mice (vitamin D3 concentration under detection level); filled triangles, high vitamin D (H-VitD) diet-fed mice (vitamin D3 concentration 75,000 IU/kg food).