Figure 5

Expression of cerebral dopamine neurotrophic factor (CDNF) by astrocytes and astrocyte-like NG2 cells in the substantia nigra pars reticulata (SNr). Representative merged confocal micrographs showing immunoreactivity to CDNF (green), GFAP (red) and NG2 (blue). The triple immune micrographs in the lower row are shown individually in the first three rows with possible combinations of double markers to see more details. The headings refer to mesencephalon slices that were obtained from different rats, intact (A), with 30-day 6-OHDA lesion (B), and 15 days of lesion and 15 days of transfection (C). Asterisk represents the cell whose fluorescent markers are individually displayed in the inserts. Graphs showing the mean fluorescence intensity (MFI) for NG2 (D), GFAP (E) and CDNF (F). The scale bars = 20 μm are common for all the micrographs. ns = not significant. ***P < 0.001 when compared with the intact condition. £ = P < 0.001 when compared with L30. One-way ANOVA with Newman-Keuls post hoc test. n = 4 different rats for each condition. GFAP, glial fibrillary acidic protein; NG2, Neuron/Glial type 2.