Figure 6

Nitrosative and oxidative stress markers in the substantia nigra (SN). Representative merged confocal micrographs showing the presence of inducible nitric oxide synthase (iNOS) + cells (green) with tyrosine hydroxylase (TH) + cells (red) in first row, with NG2+ cells (red) in the second row, with GFAP+ cells (red) in the third row, and with OX42+ cells (red) in the last row in intact (A), in L30 (B) and in L15T15 (C) conditions. Graphs showing the mean fluorescence intensity (MFI) for iNOS (D), the levels of nitrites (E) and malondialdehyde (F). The headings for micrograph panels and x-axis labels for graphs refer to mesencephalon slices that were obtained from different rats, intact, with 30-day 6-hydroxydopamine (6-OHDA) lesion, L30, and 15 days of lesion and 15 days of transfection, L15T15. The scale bars = 20 μm are common for all the micrographs. ns = not significant. *P < 0.05, **P < 0.01, ***P < 0.001 when compared with the intact condition. † = P < 0.01, £ = P < 0.001 when compared with L30. One-way ANOVA with Newman-Keuls post hoc test. n = 4 different rats for each condition. GFAP, glial fibrillary acidic protein; NG2, Neuron/Glial type 2.