Figure 8

Expression levels of total cerebral dopamine neurotrophic factor (CDNF) in the striatum. Representative merged confocal micrographs showing immunoreactivity to CDNF (green), GFAP (red) and NG2 (blue). The triple immune micrographs in the lower row are shown individually in the first three rows with possible combinations of double markers to see more details in intact (A), in L30 (B) and in L15T15 (C) conditions. Graphs showing rCDNF transcripts by qRT-PCR (D) and protein levels of rCDNF (E) and hCDNF (F) by ELISA. The headings for micrograph panels and x-axis labels for graphs refer to mesencephalon slices that were obtained from different rats, intact, with 30-day 6-hydroxydopamine (6-OHDA) lesion, L30, and 15 days of lesion and 15 days of transfection, L15T15. The scale bars = 20 μm are common for all the micrographs. ns = not significant. ***P < 0.001 when compared with the intact condition. £ = P < 0.001 when compared with L30. One-way ANOVA with Newman-Keuls post hoc test. n = 4 different rats for each condition. GFAP, glial fibrillary acidic protein; NG2, Neuron/Glial type 2.