Figure 3

Helminth soluble factor inhibits agonist induced Ca ²⁺ release and Ca ²⁺ entry. Fura-2 acetoxymethyl ester fluorescence measurements in microglia. (A) Cells were pulsed with medium alone, lipopolysaccharide (LPS) at 10 ng/ml (Control) or pretreated helminth soluble factor (HSF) at 25 μg/ml for 20 minutes before the addition of LPS to the medium (+HSF). Average analog plots of the fluorescence ratio (340/380 nm) from an average of 40 to 50 cells are shown. (B) The bar graph indicates the average data for Ca²⁺ release (left peak) and store-operated Ca²⁺ entry (SOCE) (right peak) under these conditions in microglia pulsed with LPS or HSF + LPS. Asterisks indicate significance (**P ≤ 0.01). (C) Cells were pulsed with medium alone, thapsigargin (Tg) at 1 μM or pretreated HSF at 25 μg/ml for 20 minutes before the addition of Tg at 1 μM. Analog plots of the fluorescence ratio (340/380 nm) from an average of 30 to 40 cells are shown. (D) The bar graph indicates the average data on Ca²⁺ release (left peak) and Ca²⁺ entry (right peak) under these conditions in microglia pulsed with Tg or HSF + Tg. Asterisks indicate significance (*P ≤ 0.05, ** P ≤ 0.01).