Figure 2
From: Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4
Normalization of M2 gene expression using microglial gene C1qA. At 8 h after intracerebroventricular injection of saline (Veh, open bars) or IL4 (IL4, black bars), RNAs from the frontal cortex and striatum were analyzed by Real Time PCR to evaluate Fizz1 (A) and Ym1 (B) gene expression. ΔCt values are calculated with respect to either the 36B4 housekeeping gene (versus 36B4) or C1qA microglia-specific gene (versus C1qA) and are shown as the 2-ddCt method with respect to the mean value of the vehicle group. Bars represent mean values ± SEM (n = 4 to 6).