Figure 5
From: Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4
Microglial localization and distribution of Ym1 protein in brain cells. Brains after 16 h intracerebroventricular treatment with vehicle (A,D,F) and IL4 (B,C,E,G) were analyzed by co-immunostaining using Ym1 antibody (A-G), here observed as red signals, together with cell specific antibodies Mac-1 (A-C), NeuN (D-E), and GFAP (F,G), revealed in green. Images were taken in the frontal cortex (Fr. cortex, A,B,D-G) or striatum (C). The Ym1 signal specifically co-localized with Mac-1-positive cells, highlighted by white arrows and higher magnification inserts in B and C. Bar, 30 μm; dashed bar, 8 μm.