Figure 1

Expression profiles of the nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome components following status epilepticus (SE). (A-C) Western blot assay for the expression profiles of cleaved IL-1β (18 kDa) (A), NLRP3 (106 kDa) (B) and cleaved caspase-1 (20 kDa) (C) in the hippocampus of sham rat at 12 hours following the sham operation of amygdala stimulation (without any electrical stimulation) and SE rat at 3, 6, 12, and 24 hours following amygdala stimulation. Data are expressed as a fold change relative to sham group. n = 6 rat per group and per time point. Error bars represent mean ± standard deviation. *P < 0.05 versus sham control group. NS: not significant versus 12 hours following SE. (D) Representative photographs of immunofluorescence staining for NLRP3 (green) expression in microglia (Iba-1, red) in the hippocampal area 12 hours following SE. Scale bars: 100 μm.