Caspase-1 inhibition ameliorated neuroinflammation and hippocampal neuronal loss in status epilepticus (SE) rat. (A) The expression level of cleaved IL-1β (18 kDa) was detected by Western blot. β-actin was used as loading control. Data are expressed as a fold change relative to SE rat infused with control siRNA. *P < 0.05 versus control siRNA treated group. (B) Neuronal death was detected using the TUNEL staining in the CA1 and CA3 region of the hippocampus. Photos were converted to black and white to obtain a better contrast ratio. Neurons with deep black nuclei were identified as TUNEL-positive neurons (indicated by red arrows). Scale bars: 50 μm. The percentage of TUNEL-positive neurons was defined as follows: 100× (Count of TUNEL-positive neurons/Total count of neurons). All data are shown as mean ± standard deviation (n = 6 per group). *P < 0.05 versus control siRNA-treated group. TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP end-labeling.