Figure 1

Pretreatment of ouabain (OUA) (1.8 μg/ kg) in lipopolysaccharide (LPS) (200 μg/ kg ) inflammation model induced no changes on Na,K-ATPase (NKA) activity. The total NKA activity was tested by adding 10 μg of the particulate fraction from the rat hippocampus obtained 2 hours after OUA (1.8 μg/kg, ip) or saline pretreatment + LPS (200 μg/kg, ip). α_2/3-NKA activity was measured by subtracting the activity obtained with 3 μM OUA (it inhibits just these 2 isoforms) from total NKA activity. To determine the α₁ subunit-associated NKA activity, the total NKA activity was subtracted from the activity obtained with 3 mM OUA (Mg-ATPase). Results are expressed as nmol/mg.min protein (mean + SEM) from five individual experiments. One-way ANOVA followed by Newman-Keuls post-test.