Figure 2

Effects of pretreatment of ouabain (OUA) (1.8 μg/ kg) in the lipopolysaccharide (LPS) (200 μg/ kg) inflammation model on the mRNA levels of Il - 1β , iNos , Bax , and Bcl2 . Pretreatment of OUA can decrease the mRNA levels of pro-inflammatory cytokines and pro-apoptotic proteins. Representative semiquantitative RT- PCR photographs and densitometric analysis (arbitrary units, AU) of the specific bands. mRNA levels are presented as ratios of the target gene to Gapdh expression. Data are presented as mean ± SEM from five individual experiments. One-way ANOVA followed by Newman-Keuls post-test. (A) Il-1β mRNA,*P < 0.05 versus control and OUA and **P < 0.01 versus control and OUA and OUA + LPS. (B) iNos mRNA, *P < 0.05 versus control and OUA + LPS. (C) Bax/Bcl2 ratio,**P < 0.001 versus OUA, control and OUA + LPS and *P < 0.05 versus OUA.