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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Differences in the distribution, phenotype and gene expression of subretinal microglia/macrophages in C57BL/6N (Crb1rd8/rd8) versus C57BL6/J (Crb1wt/wt) mice

Figure 2

Central fundus spots increase with age in both B6 groups, most prominently in rd8/rd8 mice. (A) A retinal pigment epithelium (RPE) flat mount of an old rd8 mouse stained for ionized calcium binding adaptor (Iba)-1 is shown to demonstrate the area counted as the ‘central flat mount’ (large white square). The ‘central flat mount’ is made up of four higher magnification photos (magnification 10X/1.6X optivar) taken around the disc (smaller squares). (B) One of those higher magnification photos is shown. (C) The number of yellow spots (black bars) in the central fundus (within a circle with a 5 disc diameter (DD) radius and centered on the disc) is very similar to the number of Iba-1+ cells (gray bars) on the corresponding central flat mounts. There is an increase in central fundus spots in rd8/rd8 mice compared to wild-type (WT) mice in each age group. Furthermore, there is an increase in the number of central yellow spots with age in both genotypes (young WT (n = 11), young rd8 (n = 3); old WT (n = 11), old rd8 (n = 10)). (D) Linear regression showing a significant correlation between the number of yellow spots in central fundus (5 DD radius) and the number of Iba-1 positive cells in the corresponding flat mount area (n = 19 eyes). Mice 2 to 8 months of age were classified as ‘young’, while mice 14 to 20 months of age were classified as ‘old’. *P <0.05, ***P <0.001.

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