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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Differences in the distribution, phenotype and gene expression of subretinal microglia/macrophages in C57BL/6N (Crb1rd8/rd8) versus C57BL6/J (Crb1wt/wt) mice

Figure 3

The total number of subretinal microglia/macrophages (MG/MΦ) increases with age in C57BL/6N, but not C57BL/6J mice. (A) Number of ionized calcium binding adaptor (Iba)-1 positive cells in the central flat mount of young and old mice showing a significant increase in both age groups of rd8/rd8 mice compared to the age-matched wild-type (WT). (B) Number of Iba-1 positive cells in the entire/total retinal pigment epithelium (RPE) flat mounts showing that the total number of subretinal microglia increases with age in rd8/rd8 mice, but not in WT mice. The graphs represent the combined results of two to three experiments, which included young mice (WT, n = 10 eyes; or rd8/rd8, n = 8 eyes), and old mice (WT, n = 13 eyes; or rd8/rd8, n = 11 eyes). Mice 2 to 8 months of age were classified as ‘young’, while mice 14 to 20 months of age were classified as ‘old’. *P <0.05, **P <0.01, ***P <0.001.

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