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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Differences in the distribution, phenotype and gene expression of subretinal microglia/macrophages in C57BL/6N (Crb1rd8/rd8) versus C57BL6/J (Crb1wt/wt) mice

Figure 4

The phenotype of subretinal microglia/macrophages in B6-mice changes with age and with the rd8 mutation. (A-F) Representative images of triple-stain immunohistochemistry (IHC) show staining with ionized calcium binding adaptor (Iba)-1 (A and D), Macrophage mannose receptor (MMR) (B and E), and FcγIII/II Receptor (CD16/CD32, abbreviated as CD16) (C and F) of subretinal microglia/macrophages (MG/MΦ) on retinal pigment epithelium (RPE)-flat mounts. Different staining patterns are shown here: some Iba-1+ cells stain strongly for CD16 (D and F), while some Iba-1+ cells show weak or no staining for CD16 (A and C). (G) Quantification of MMR + CD16- MG/MΦ in the entire RPE-flat mount shows that in old mice, there is a significant increase in MMR + CD16- cells in rd8/rd8 mice compared to wild-type (WT) mice. (H) In the entire flat mount, there is also a significant increase in the number of CD16+ subretinal MG/MΦ in old rd8/rd8 mice compared to old WT. (I) The central flat mounts demonstrate a significant increase in CD16+ MG/MΦ in old rd8/rd8 mice compared to old WT mice. There is also a trend towards an increase in the number of MMR + CD16- cells in the central flat mounts of old rd8/rd8 mice compared to WT. For figures G-I we combined three similar experiments using 10 young WT, 8 young rd8/rd8, 13 old WT and 10 old rd8/rd8 eyes. Mice 2 to 8 months of age were classified as ‘young’, while mice 14 to 20 months of age were classified as ‘old’. *P <0.05, ***P <0.001, #P = 0.056.

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