Figure 6

Subretinal microglia/macrophages (MG/MΦ) in C57BL/6N mice express markers of activation. After processing eyes using the freeze-substitution technique (see Methods, n = 8 eyes per group), retina sections from C57BL/6N (A-D and F-I) or from C57BL/6J (E and J) eyes were obtained and stained. Each section was double-labeled with ionized calcium binding adaptor (Iba)-1 (red channel; G-J) and one microglia/macrophage activation marker (green channel; B-E). A control sample is also shown (secondary antibodies without primary antibodies; A and F), which is a consecutive section of the one stained with Iba-1 and FcγIII/II Receptor (CD16/CD32, abbreviated as CD16) (B and G). Note the expression of CD16 and activation markers MHC-II and iNOS in subretinal MG/MΦ in rd8 mutant samples (arrows in B,C,D). There is no staining for MHC-II in the wild-type (WT) subretinal MG/MΦ (E versus C).