Figure 8

RT-qPCR analysis of RNA from retinal pigment epithelium (RPE)/subretinal microglia/macrophages (MG/MΦ) isolates and from retina isolates. There is an upregulation of genes related to oxidative stress, complement activation and inflammation in rd8 mutant mice compared to wild-type (WT). (A) Fold changes in the expression of target genes, in RNA isolated from RPE/subretinal MG/MΦ from rd8 mutant mice, normalized to B6-WT. The bars represent the average of two experiments in age-matched 6- to 8-month-old mice, including a total of 10 rd8/rd8 and 10 WT eyes, except for CFB (6 rd8/rd8 and 6 WT eyes). (B) Fold changes in the expression of target genes in RNA isolated from the retina of rd8 mutant mice normalized to B6-WT. The bars represent the average of two experiments including a total of 11 rd8/rd8 and 11 B6 eyes. *P <0.05, **P <0.01, ***P <0.001, #P = 0.058.