Figure 7

Lipopolysaccharide (LPS) reduces the amount of gamma-amino butyric acid (GABA) release from presynaptic neurons. (A) shows electrically evoked GABAergic evoked inhibitory postsynaptic currents (eIPSCs) at baseline, in the presence of tetrahydropyridin-4-yl methylphosphinic acid (TPMPA) (60 μM), and after TPMPA washout. (B) shows eIPSCs in the presence of LPS (1 μg/ml), in the presence of LPS (1 μg/ml) plus TPMPA (60 μM) and washout of both TPMPA and LPS. Note data in (A) and (B) were recorded from the same neuron. The eIPSCs were recorded with a recording pipette filled with the intracellular solution containing the PKC inhibitor (PKCI 19–30, 5 μM). The bar graph show mean (+SE) percentages of eIPSC amplitudes during perfusion of TPMPA in the absence of LPS (second bar to the left), and in the presence of LPS (the far right bar). Amplitudes prior to TPMPA perfusion in the absence (the far left bar) or presence of LPS (the second bar to the right) were used as the baseline for calculating the percentages. **P < 0.01; ***P < 0.001.