Figure 9

Inhibition of protein kinase C (PKC) or IL-1β receptors blocks the inhibitory effects induced by lipopolysaccharide (LPS) on GABAergic synaptic activities at the presynaptic site. A sample of GABAergic miniature inhibitory postsynaptic currents (mIPSCs) recorded in the presence of the IL-1β receptor blocker (IL-1ra, 100 ng/ml) (A) or PKC inhibitor (GF109203X, GF) (B) before, during, and after washout of LPS (1 μg/ml). Bar graphs show that mean (+SE) frequencies and amplitudes of GABAergic mIPSCs before (baseline), during perfusion of IL-1ra or GF109203X and further addition of LPS in the presence of IL-1ra (C) or GF109203X (D). NS: no statistical significance.