Regulation by C/EBPβ of the activity of the mouse
promoter. (A) RT-PCR analysis of C3 mRNA content in the hippocampus of C/EBPβ
+/+ and C/EBPβ
−/− mice. The graphic shows the mean ± SD of three different experiments. **P <0.01. (B, C) Analysis, by transient transfection in GL261 cells, of the effect of different deletions (B) and point mutations (C) on the activity of C3 promoter. Data represent the mean ± SD of luciferase activity determined in triplicate in at least three independent experiments. ***P <0.001 versus whole (B) or non-mutated (C)
C3 promoter; #P <0.05, ###P <0.001 versus C3 basal promoter activity. (D) Representative image of ChIP analysis of C/EBPβ binding to the endogenous C3 promoter in primary astrocytes. DNA before immunoprecipitation was used as positive control (Input).