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Figure 3 | Journal of Neuroinflammation

Figure 3

From: CCAAT/enhancer binding protein β directly regulates the expression of the complement component 3 gene in neural cells: implications for the pro-inflammatory effects of this transcription factor

Figure 3

Effect of C/EBPβ and C3 on IL-1β and COX-2 gene expression in mouse astrocytes. Astrocytes were transiently transfected with the indicated vectors or oligonucleotides, treated or not with lipopolysaccharide (LPS), and 24 h later the content of C/EBPβ, C3, IL-1β and COX2 proteins analyzed as indicated in Methods. (A) Representative western blot showing expression of C/EBPβ and C3 in astrocytes overexpressing C/EBPβ (pcDNA3β). Quantification analysis is shown. Data represent the mean ± SD of five different experiments. *P <0.05, ***P <0.001 versus basal values. (B) Representative western blot showing expression of C/EBPβ, C3, IL-1β, and COX-2 in astrocytes transfected with a C/EBP shRNA and treated or not with LPS. Quantification analysis is shown. Values represent the mean ± SD of five different experiments. *P <0.05, **P <0,01, ***P <0.001 versus shRNA Ctrl. (C) Representative Western blot showing expression of C3, IL-1β, and COX-2 in astrocytes transfected with C3 siRNA and treated or not with LPS. Quantification analysis is shown. Values represent the mean ± SD of five different experiments. (D) Astrocytes were transfected with C3 siRNA or a non-targeting siRNA (siRNA Ctrl), treated or not with LPS and nitrite production was measured as indicated in Methods. ***P <0.001 versus siRNA Ctrl + LPS.

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